Assume that the reaction catalyzed by an enzyme follows Michaelis-Menten kinetics. The substrate concentration (Km, Michaelis constant) needed to reach 50% of the maximum reaction velocity (Vmax) is 25 µM. What substrate concentration is required to obtain at least 50% of the maximum reaction velocity?

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Physiology Test Question

Assume that the reaction catalyzed by an enzyme follows Michaelis-Menten kinetics. The substrate concentration (K_m, Michaelis constant) needed to reach 50% of the maximum reaction velocity (V_max) is 25 μM. What substrate concentration is required to obtain at least 50% of the maximum reaction velocity?

	(A) 25 μM
	(B) 50 μM
	(C) 100 μM
	(D) 250 μM
	(E) 475 μM

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Posted: Friday, December 12, 2014
Last updated: Friday, December 12, 2014