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Dilution Factor Calculator - Cells per Volume

Meant to be used in both the teaching and research laboratory, this calculator (see below) can be utilized to perform **dilution factor** calculations when working with solutions having **cells per volume** (i.e., cells over volume) concentration units such as cells/mL, cells/L, 10^{3} cells/mL, 10^{6} cells/L, etc. These calculations are commonly performed when working with culture media containing living cells such as bacterial cells or mammalian cells. See also our Cells per Volume Dilution Calculator. When a concentrated solution is diluted, the dilution factor may be expressed as the ratio of the concentration of stock solution to the concentration of the diluted solution. The dilution factor may also be expressed as the ratio of the volume of the final diluted solution to the initial volume removed from the stock solution. See below for the dilution factor equations.

For example, if a solution with a concentration of 1 × 10^{6} cells/mL is diluted to yield a solution with a concentration of 1 × 10^{3} cells/mL, the resulting dilution factor is 1000. For this particular dilution, it can also be said that the stock solution was diluted 1000-**fold**. As another example, if 100 mL of a stock solution is diluted with solvent/diluent to a total, final volume of 1000 mL, the resulting dilution factor is 10. It can also be said that the stock solution was diluted 10-**fold**. Therefore, a 10-fold dilution is the same as a dilution factor of 10. Thus, dilution by any factor *X* is equivalent to *X*-fold dilution.

Please note that the dilution factor calculator below uses the metric unit for volume (fL, pL, nL, μL, mL, and L). If you wish to convert these units to other volume units, please use our Unit Conversion Calculator.

Additional dilution factor calculators are also available and are suited to more specialized applications (see here).

Dilution factor equation - cells per volume

is the factor by which the stock solution is diluted. It may be expressed as the ratio of the volume of the final diluted solution (*Dilution Factor**V*_{2}) to the initial volume removed from the stock solution (*V*_{1}), as shown in the equation above. Dilution factor may also be expressed as the ratio of the concentration of stock solution (*C*_{1}) to the concentration of the final diluted solution (*C*_{2}).is the concentration of the stock solution.*Stock Solution Concentration (C*_{1})is the volume to be removed (i.e., aliquoted) from the concentrated stock solution.*Volume from Stock Solution (V*_{1})is the concentration of the final diluted solution.*Final Diluted Solution Concentration (C*_{2})is the final volume of the diluted solution. This is the volume that results after the volume from the stock solution (*Final Solution Volume (V*_{2})*V*_{1}) has been diluted with solvent or diluent to achieve a total diluted volume of the final solution (*V*_{2}). Please note that the final volume refers to the total solution volume, which is the combined volume of the stock solution and the volume of solvent/diluent used for dilution.

Dilution factor calculator - cells per volume (cells / volume)

Each calculator cell shown below corresponds to a term in the formula presented above. Two main options are available for this calculator: the dilution factor may either be calculated or specified.

For convenience, this calculator allows you to select different volume and concentration units, and the necessary conversions are carried out for you to obtain the value of the blank cell(s) in the desired unit.

Comments and/or preparation instructions

Relevant comments and/or instructions will appear here after a calculation is performed.

Posted: Sunday, February 10, 2019

Last updated: Saturday, February 29, 2020

Last updated: Saturday, February 29, 2020