The action potential is a rapid and reversible reversal of the electrical potential difference across the plasma membrane of excitable cells such as neurons, muscle cells and some endocrine cells. In a neuronal action potential, the membrane potential rapidly changes from its resting level of approximately -70 mV to around +50 mV and, subsequently, rapidly returns to the resting level again. The neuronal action potential forms an important basis for information processing, propagation, and transmission. In muscle cells, the action potential precedes, and is necessary to bring about, muscle contraction. Some endocrine cells also exhibit action potentials, where the excitation leads to hormone secretion.

The action potential is also referred to as the electrical impulse or nervous impulse.

Graded potential

Neuronal Action Potential

Refers to the hyperpolarization phase of the action potential.

It is also referred to as undershoot.

See figure.

Hyperpolarization

Important Features of the Neuronal Action Potential

The voltage difference across a cell plasma membrane.

The membrane potential is generally inside negative with respect to the outside, where the outside potential is generally set as the reference value. In electrically excitable cells, the value of the membrane potential can be positive (inside with respect to the outside) during electrical activity (i.e., during action potentials).

Resting membrane potential

Resting membrane potential

The absolute refractory period refers to a period during the action potential. This is the time during which another stimulus given to the neuron (no matter how strong) will not lead to a second action potential. The absolute refractory period starts immediately after the initiation of the action potential and lasts until after the peak of the action potential. Following this period, the relative refractory period begins.

Relative refractory period

Neuronal Action Potential - Refractory Periods

All-or-nothing is usually used when describing the action potential. It refers to the well-known observation that an action potential always occurs in its full size (i.e., full magnitude of voltage change).

Many physiologists use all-or-nothing and all-or-none interchangeably.

Important Features of the Neuronal Action Potential

The region of the neuron cell body from which the axon originates. The axon hillock is generally the site of action potential initiation. It is also referred to as the initial segment.

CI⁻

The main anion (negatively charged ion) of the extracellular fluid.

Cloride (Cl⁻) plays an important role in several physiological processes such as the action potential of skeletal muscle cells, CO₂ transport in blood (via Cl⁻/bicarbonate exchange across the plasma membrane of red blood cells), and many other processes.

The extracellular concentration of Cl⁻ is about 110 mM. The intracellular concentration of Cl⁻ is about 10 mM.

Electrophysiology is the study of the electrical properties of biological macromolecules, cells, tissues, and organs. Electrical signals such as voltage and/or current are generally measured. Examples include measuring changes in the membrane voltage of excitable cells (e.g., neurons, muscle cells, and some endocrine cells) during an action potential. The current carrried by ions as they permeate the pore of ion channels can also be measured - both at the single-channel level (single-channel current), as well as the macroscopic current resulting from the activity of a population of channels. As another example, electrical measurements may involve recording voltage changes at the surface of the skin that result from the activity of skeletal muscles (electromyogram, EMG), cardiac myocytes (electrocardiogram, ECG), or neurons in the brain (electroencephalogram, EEG).

Refers to the ability of some cells to be electrically excited resulting in the generation of action potentials. Neurons, muscle cells (skeletal, cardiac, and smooth), and some endocrine cells (e.g., insulin-releasing pancreatic β cells) are excitable cells.

Resting Membrane Potential - Introduction

Refers to the action potential of neurons. It is also referred to as the nervous impulse.

Action potential

Neuronal Action Potential

Refers to the action potential of neurons. It is also referred to as the nerve impulse.

Action potential

Neuronal Action Potential

Refers to cells that do not generate action potentials. With the exception of neurons, muscle cells, and some endocrine cells, all cells in the body are non-excitable.

Resting Membrane Potential - Introduction

Refers to that part of the action potential where the membrane potential is positive (inside with respect to the outside).

See figure.

Neuronal Action Potential - Important Features of the Neuronal Action Potential

K⁺

The main cation (positively charged ion) of the intracellular fluid.

Potassium (K⁺) plays an important role in the action potential of neurons and muscle cells.

The extracellular concentration of K⁺ is about 4 mM. The intracellular concentration of K⁺ is about 150 mM.

The relative refractory period refers to a period during the action potential. This is the time during which a stronger than normal stimulus is needed in order to elicit an action potential. The relative refractory period immediately follows the absolute refractory period.

Absolute refractory period

Neuronal Action Potential - Refractory Periods

Na⁺

The main cation (positively charged ion) of the extracellular fluid.

Sodium (Na⁺) plays an important role in several physiological processes such as the action potential of neurons and muscle cells, secondary active, sodium-coupled transport of ions, nutrients, neurotransmitters across the plasma membrane of cells, and many other processes.

The extracellular concentration of Na⁺ is about 145 mM. The intracellular concentration of Na⁺ is about 15 mM.

Refers to the rapid depolarization of the membrane early in the action potential. In neuronal, skeletal muscle, and cardiac muscle action potentials, the Hodgkin cycle is responsible for the spike phase of the action potential.

See figure.

Important Features of the Neuronal Action Potential

Action potential

Action potential

Sub-threshold (or subthreshold) refers to a stimulus that is too small in magnitude to produce an action potential in excitable cells.

In general, a sub-threshold stimulus leads to the depolarization of the membrane, but the magnitude of the depolarization is not large enough to reach the threshold voltage. Therefore, sub-threshold stimuli do not elicit action potentials.

Threshold
Supra-threshold

Neuronal Action Potential - Introduction

Supra-threshold (or suprathreshold) refers to a stimulus that is large enough in magnitude to produce an action potential in excitable cells.

In general, a supra-threshold stimulus leads to the depolarization of the membrane, and the magnitude of the depolarization is larger than that necessary to simply reach the threshold voltage. Therefore, supra-threshold stimuli elicit action potentials.

Threshold
Sub-threshold

Neuronal Action Potential - Introduction

The membrane voltage that must be reached in an excitable cell (e.g., neuron or muscle cell) during a depolarization in order to generate an action potential. At the threshold voltage, voltage-gated channels become activated. Threshold is approximately −50 to −40 mV in most excitable cells.

Sub-threshold
Supra-threshold

Neuronal Action Potential - Introduction

Refers to the hyperpolarization phase of the action potential.

It is also referred to as hyperpolarizing afterpotential.

See figure.

Hyperpolarization

Important Features of the Neuronal Action Potential