PhysiologyWeb Logo  Search

PhysiologyWeb Loading...

Dilution Calculator - Molarity, Percent
Calculators for preparing physiological and biochemical solutions
Meant to be used in both the teaching and research laboratory, this calculator (see below) can be utilized to perform dilution calculations when working with molar or percent (%) solutions. When working with % solutions, the concentration unit must be % for both the stock concentrated solution and final diluted solution. Additional dilution calculators are also available and are suited to more specialized applications (see here).
If you are starting with the solid material and wish to make a solution with the concentration expressed in molarity, use our Molar Solution Concentration Calculator.
If you are starting with the solid or liquid material and wish to make a weight/volume %, weight/weight %, or volume/volume % solution, use our Percent Solution Concentration Calculator (w/v %, w/w %, and w/v %).
Dilution equation

Dilution Equation

  • C1 is the concentration of the stock solution.
  • V1 is the volume to be removed (i.e., aliquoted) from the concentrated stock solution.
  • C2 is the final concentration of the diluted solution.
  • V2 is the final volume of the diluted solution. This is the volume that results after V1 from the stock solution has been diluted with diluent to achieve a total diluted volume of V2.
  • An alternative and commonly-used notation for this equation is M1V1 = M2V2, where M is used in place of C.
Dilution calculator - molarity, percent
Each calculator cell shown below corresponds to a term in the formula presented above. Enter appropriate values in all cells except the one you wish to calculate. Therefore, at least three cells must have values, and no more than one cell may be blank. The value of the blank cell will be calculated based on the other values entered. After a calculation is performed, the calculated cell will be highlighted and subsequent calculations will calculate the value of the highlighted cell (with no requirement to have a blank cell). However, a blank cell has priority over a highlighted cell.
For convenience, this calculator allows you to select different volume and concentration units, and the necessary conversions are carried out for you to obtain the value of the blank cell in the desired unit.
Stock concentration mM
Volume from stock mL
Final concentration mM
Final solution volume mL
        
Comments and/or preparation instructions
Relevant comments and/or instructions will appear here after a calculation is performed.



Posted: Thursday, March 1, 2001
Last updated: Sunday, March 2, 2014