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Dilution Calculator - Cells per Volume

Meant to be used in both the teaching and research laboratory, this calculator (see below) can be utilized to perform dilution calculations when working with solutions having **cells per volume** (i.e., cells over volume) concentration units such as cells/mL, cells/L, 10^{3} cells/mL, 10^{6} cells/L, etc. These calculations are commonly performed when working with culture media containing living cells such as bacterial cells or mammalian cells. Additional dilution calculators are also available and are suited to more specialized applications (see here).

Dilution equation

is the concentration of the stock solution.*C*_{1}is the volume to be removed (i.e., aliquoted) from the concentrated stock solution.*V*_{1}is the final concentration of the diluted solution.*C*_{2}is the final volume of the diluted solution. This is the volume that results after*V*_{2}*V*_{1}from the stock solution has been diluted with diluent to achieve a total diluted volume of*V*_{2}.- An alternative and commonly-used notation for this equation is
*M*_{1}*V*_{1}=*M*_{2}*V*_{2}, where*M*is used in place of*C*.

Dilution calculator - cells / volume

Each calculator cell shown below corresponds to a term in the formula presented above. Enter appropriate values in all cells except the one you wish to calculate. *Therefore, at least three cells must have values, and no more than one cell may be blank*. The value of the blank cell will be calculated based on the other values entered. After a calculation is performed, the calculated cell will be highlighted and subsequent calculations will calculate the value of the highlighted cell (with no requirement to have a blank cell). However, a blank cell has priority over a highlighted cell.

For convenience, this calculator allows you to select different volume and concentration units, and the necessary conversions are carried out for you to obtain the value of the blank cell in the desired unit.

Please note that the calculator below does not accept values expressed using the scientific notation (e.g., 1 × 10^{6}). Instead, please use either the standard decimal notation or the E notation. For example, 1 × 10^{6} may be expressed as 1000000 (standard decimal notation) or 1e6 (E notation).

Comments and/or preparation instructions

Relevant comments and/or instructions will appear here after a calculation is performed.

Posted: Saturday, April 9, 2005

Last updated: Tuesday, June 28, 2011

Last updated: Tuesday, June 28, 2011